1. Pokémon Diamond / Pearl ( 5,578,782 )
2. New Super Mario Bros. ( 5,281,886 )
3. Brain Age 2: More Training in Minutes a Day ( 4,897,082 )
4. Animal Crossing: Wild World ( 4,730,152 )
5. Brain Age: Train Your Brain in Minutes a Day ( 3,689,161 )
6. Mario Kart DS ( 3,216,022 )
7. English Training: Have Fun Improving Your Skills ( 2,051,037 )
8. Nintendogs (Labrador, Dachshund, Chihuahua) ( 1,777,272 )
9. Mario Party DS ( 1,763,496 )
10. Pokémon Platinum ( 1,602,535 )
11. Kanshuu Nippon Joushikiryoku Kentei Kyoukai: Imasara Hito ni wa Kikenai Otona no Joushikiryoku Training DS ( 1,538,618 )
12. Big Brain Academy ( 1,492,095 )
13. Dragon Quest Monsters: Joker ( 1,458,149 )
14. Pokemon Mystery Dungeon Time/Darkness ( 1,392,972 )
15. Tetris DS ( 1,232,061 )
16. Dragon Quest IV ( 1,201,771 )
17. Dragon Quest V ( 1,152,229 )
18. WarioWare: Touched! ( 1,136,255 )
19. Tamagotchi Connection: Corner Shop ( 1,110,046 )
20. Super Mario 64 DS ( 1,069,060 )
21. Kirby Squeak Squad ( 1,060,257 )
22. Oshare Majo Love & Berry – DS Collection ( 1,039,669 )
23. Final Fantasy III ( 1,008,230 )
